6Q87
Structure of Fucosylated D-antimicrobial peptide SB10 in complex with the Fucose-binding lectin PA-IIL at 2.541 Angstrom resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-07-06 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.000031 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 130.200, 130.200, 49.623 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.419 - 2.541 |
| R-factor | 0.2147 |
| Rwork | 0.212 |
| R-free | 0.25730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oxc |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.923 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.7.16) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.419 | 2.701 |
| High resolution limit [Å] | 2.540 | 2.541 |
| Rmeas | 0.076 | |
| Number of reflections | 15464 | |
| <I/σ(I)> | 12.74 | |
| Completeness [%] | 99.4 | 98 |
| Redundancy | 6.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 5% v/v Tacsimate pH 7.0, 0.1 M HEPES pH 7.0 ,10% w/v Polyethylene glycol monomethyl ether 5,000 |
