6Q82
Crystal structure of the biportin Pdr6 in complex with RanGTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97796 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 192.990, 192.990, 229.170 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 59.894 - 2.994 |
| R-factor | 0.219 |
| Rwork | 0.218 |
| R-free | 0.25290 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.894 | 3.101 |
| High resolution limit [Å] | 2.994 | 2.994 |
| Rmerge | 0.273 | |
| Rpim | 0.014 | |
| Number of reflections | 51160 | 4876 |
| <I/σ(I)> | 44.7 | 1.5 |
| Completeness [%] | 99.7 | 97.42 |
| Redundancy | 399.8 | |
| CC(1/2) | 1.000 | 0.893 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.26 | 291 | Pentaerythritol propoxylate (17/8 PO/OH), MPD, Sodium acetate, Magnesium chloride |
