6Q75

The structure of GH26A from Muricauda sp. MAR_2010_75

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-09
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0332
Spacegroup name	P 1
Unit cell lengths	47.524, 49.342, 71.711
Unit cell angles	78.00, 89.83, 69.64

Refinement procedure

Resolution	44.428 - 1.750
R-factor	0.1541
Rwork	0.152
R-free	0.18990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wdq
RMSD bond length	0.009
RMSD bond angle	0.932
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.120	1.840
High resolution limit [Å]	1.750	1.750
Rmerge	0.089	0.482
Number of reflections	58106	8362
<I/σ(I)>	9.2	2.7
Completeness [%]	96.7	95.2
Redundancy	4.3
CC(1/2)	0.996	0.885

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.15M Magnesium chloride 0.1M TRIS pH 8.5 21% PEG4000 20% Glycerol