6Q6P
Antarctic fish cytoglobin 1 from D.mawsoni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 70 |
| Detector technology | PIXEL |
| Collection date | 2017-02-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.658, 105.647, 127.328 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.530 - 3.000 |
| R-factor | 0.19235 |
| Rwork | 0.189 |
| R-free | 0.24511 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1umo |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.468 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.300 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.242 | 0.784 |
| Number of reflections | 12427 | |
| <I/σ(I)> | 8.7 | 4.2 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 12.3 | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 2 M ammonium sulphate, 0.1 M Tris |
