6Q69

Crystal structure of porcine ACBD3 GOLD domain in complex with 3A protein of enterovirus-G1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 1
Unit cell lengths	54.846, 55.767, 61.163
Unit cell angles	96.18, 105.54, 112.82

Refinement procedure

Resolution	30.156 - 2.747
R-factor	0.2305
Rwork	0.228
R-free	0.27080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lz1
RMSD bond length	0.003
RMSD bond angle	0.808
Data reduction software	XDS (Jan 26, 2018)
Data scaling software	XDS (Jan 26, 2018)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.160	2.846
High resolution limit [Å]	2.747	2.747
Rmerge	0.081	0.602
Rmeas	0.109
Number of reflections	14434	1306
<I/σ(I)>	8.37	1.38
Completeness [%]	88.5	80.62
Redundancy	2.1	2.1
CC(1/2)	0.997	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	12,5% w/v PEG 4000, 20% v/v 1,2,6-hexanetriol, 30mM lithium sulfate, 30mM sodium sulfate, 30mM pottasium sulfate, 100mM GlyGly/AMPD pH 8,5