6Q68
Crystal structure of bovine ACBD3 GOLD domain in complex with 3A protein of enterovirus-F2 (fusion protein)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 43 |
| Unit cell lengths | 55.371, 55.371, 200.861 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.490 - 3.161 |
| R-factor | 0.243 |
| Rwork | 0.241 |
| R-free | 0.27390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lz1 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.774 |
| Data reduction software | XDS (Jan 26, 2018) |
| Data scaling software | XDS (Jan 26, 2018) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.490 | 3.274 |
| High resolution limit [Å] | 3.161 | 3.161 |
| Rmerge | 0.078 | 1.035 |
| Rmeas | 0.084 | |
| Number of reflections | 10318 | 1049 |
| <I/σ(I)> | 16.06 | 1.47 |
| Completeness [%] | 99.8 | 99.24 |
| Redundancy | 7.5 | 7.3 |
| CC(1/2) | 0.999 | 0.662 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 15% w/v PEG 3000, 10% v/v 1,4-butanediol, 1% w/v N,N-dimethyldodecylamine-N-oxide , 10% w/v glucose, 4% v/v 1,2-propandiol, 100mM BES/TEA pH 7.5 |
