6Q5Q
Crystal structure of a CC-Hex mutant that forms an antiparallel four-helix coiled coil CC-Hex-KgEb
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2010-05-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 28.570, 29.350, 55.722 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.500 - 1.080 |
| R-factor | 0.13531 |
| Rwork | 0.135 |
| R-free | 0.14659 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.222 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | ACORN |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.470 | 1.140 |
| High resolution limit [Å] | 1.080 | 1.080 |
| Rmerge | 0.074 | 0.706 |
| Rmeas | 0.077 | 0.737 |
| Rpim | 0.021 | |
| Number of reflections | 10348 | 1461 |
| <I/σ(I)> | 17.5 | 56.2 |
| Completeness [%] | 99.7 | 94.7 |
| Redundancy | 12.8 | 12.2 |
| CC(1/2) | 0.999 | 0.943 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2M potassium/sodium tartrate tetrahydrate 20% w/v PEG 3350. |
