6Q56
Crystal structure of the B. subtilis M1A22 tRNA methyltransferase TrmK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2011-09-30 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9334 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.560, 51.980, 108.130 |
| Unit cell angles | 90.00, 110.60, 90.00 |
Refinement procedure
| Resolution | 46.239 - 2.000 |
| R-factor | 0.2136 |
| Rwork | 0.212 |
| R-free | 0.23740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ku1 |
| RMSD bond length | 0.032 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10pre_2104: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.240 | 2.175 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.211 | 1.451 |
| Number of reflections | 57747 | 4968 |
| <I/σ(I)> | 12 | 1.11 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 | 3.8 |
| CC(1/2) | 0.227 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 0.2 M ammonium acetate 0.1 M sodium acetate trihydrate pH 5 30% w/v polyethylene glycol 4000 8% 2-Methyl-2,4-pentanediol |
