6Q53
CRYSTAL STRUCTURE OF THE LIGHT-HARVESTING COMPLEX II (B800-850) FROM Ectothiorhodospira haloalkaliphila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-21 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97989 |
| Spacegroup name | P 4 3 2 |
| Unit cell lengths | 177.240, 177.240, 177.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.959 - 3.701 |
| R-factor | 0.2418 |
| Rwork | 0.240 |
| R-free | 0.27690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lgh |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.987 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.700 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Number of reflections | 11486 | |
| <I/σ(I)> | 10.8 | |
| Completeness [%] | 99.9 | |
| Redundancy | 18.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 289 | 3.5% 1,2,3-heptanetriol, 2% dioxane, 0.05% LDAO and 1 M potassium phosphate, |
