6Q42
Crystal Structure of Human Pancreatic Phospholipase A2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.07184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.610, 58.951, 122.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.000 - 1.900 |
| Rwork | 0.175 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b00 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.200 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.119 | 0.975 |
| Number of reflections | 18069 | |
| <I/σ(I)> | 8.8 | 1.9 |
| Completeness [%] | 99.3 | 98 |
| Redundancy | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 291 | 32% (w/v) PEG4K, 0.8M Lithium chloride, Tris-HCl |
