6Q3A

Apo form of Apolipoprotein N-acyltransferase (Lnt)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0
Spacegroup name	P 64 2 2
Unit cell lengths	80.725, 80.725, 442.575
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.893 - 3.100
R-factor	0.2523
Rwork	0.251
R-free	0.28650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n6l
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.893	46.892	3.200
High resolution limit [Å]	3.010	8.890	3.010
Rmerge	0.199	0.044	6.094
Rmeas	0.204	0.046	6.256
Number of reflections	31865	1254	5091
<I/σ(I)>	12.87	65.2	0.45
Completeness [%]	99.6	98	98.9
Redundancy	20.781	18.884	19.654
CC(1/2)	0.999	0.999	0.161

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	7.2	277	36 % PEG200, 400 mM Ammonium phosphate dibasic, 100 mM HEPES