6Q1Z

Crystal structure of human 1G04 Fab in complex with influenza virus neuraminidase from A/Hunan/02650/2016 (H7N9)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.03309
Spacegroup name	I 4
Unit cell lengths	180.242, 180.242, 148.125
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.365 - 3.446
R-factor	0.1995
Rwork	0.197
R-free	0.24720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l14 5itb 4fql
RMSD bond length	0.010
RMSD bond angle	1.265
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.400	3.510
High resolution limit [Å]	3.446	3.450
Rpim	0.050	0.480
Number of reflections	30994	1539
<I/σ(I)>	14.6	1
Completeness [%]	99.1	0.87
Redundancy	4.4	4.1
CC(1/2)	0.997	0.844

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES, pH 7.5, 5%(w/v) PEG3000, 30% (w/v) PEG400, 10%(v/v) glycerol