6Q1W

Coiled-coil Trimer with Glu:Val:Lys Triad

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-27
Detector	APEX II CCD
Wavelength(s)	1.5406
Spacegroup name	H 3
Unit cell lengths	38.136, 38.136, 104.497
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	17.420 - 1.498
R-factor	0.177
Rwork	0.175
R-free	0.19680
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	SADABS
Data scaling software	SADABS
Phasing software	PHENIX
Refinement software	PHENIX (dev_2906)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.420	1.552
High resolution limit [Å]	1.498	1.498
Rmerge	0.158	0.251
Rmeas	0.166	0.310
Rpim	0.052	0.178
Number of reflections	9110	886
<I/σ(I)>	18.3
Completeness [%]	99.6
Redundancy	6.1
CC(1/2)	0.924	0.909

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	40% PEG600, 100 mM sodium citrate tribasic/citric acid, pH 5.5