6Q1M

Crystal structure of the wheat dwarf virus Rep domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-14
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	.97910
Spacegroup name	P 41 21 2
Unit cell lengths	49.566, 49.566, 145.653
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.920 - 1.240
R-factor	0.1581
Rwork	0.158
R-free	0.16640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	C-alphas of a trimmed pdb ID 1l2m
RMSD bond length	0.006
RMSD bond angle	0.870
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.920	1.260
High resolution limit [Å]	1.240	1.240
Rmerge	0.050	0.952
Rmeas	0.054
Rpim	0.028
Number of reflections	52013	2612
<I/σ(I)>	18.8
Completeness [%]	98.8
Redundancy	6.5
CC(1/2)	0.999	0.525

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1M Hepes