6Q1H

Structure of P. aeruginosa ATCC27853 NucC, cAAA-bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	80.498, 80.450, 262.078
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.950 - 1.450
R-factor	0.1481
Rwork	0.147
R-free	0.16700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6p7o
RMSD bond length	0.009
RMSD bond angle	1.060
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.540
High resolution limit [Å]	1.450	1.450
Rmerge	0.083	1.261
Rmeas	0.087	1.368
Number of reflections	293192	40591
<I/σ(I)>	20.4	1.2
Completeness [%]	97.1	83.7
Redundancy	12.1	6.8
CC(1/2)	1.000	0.499

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1 M HEPES pH 7.5, 0.1 M L-proline, 10% PEG 3350