6Q18

Human antibody H1244 in complex with the influenza hemagglutinin head domain of A/Beijing/262/95(H1N1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	80
Detector technology	CCD
Collection date	2017-10-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9999
Spacegroup name	P 21 21 21
Unit cell lengths	58.000, 69.640, 191.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.608 - 2.550
R-factor	0.2077
Rwork	0.206
R-free	0.24320
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.608	2.641
High resolution limit [Å]	2.550	2.550
Rmerge	0.112	1.009
Number of reflections	26099	2560
<I/σ(I)>	17.05
Completeness [%]	99.9	99.88
Redundancy	8
CC(1/2)	0.998	0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291.15	200mM sodium chloride, 30% (v/v) PEG400, 100mM HEPES