6Q0O

human antibody H2227 lineage 652 in complex with influenza hemagglutinin head domain of A/Solomon Islands/3/2006(H1N1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	80
Detector technology	CCD
Collection date	2014-10-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 21 21 2
Unit cell lengths	61.960, 88.600, 147.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.024 - 3.000
R-factor	0.2316
Rwork	0.230
R-free	0.26770
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.024	3.107
High resolution limit [Å]	3.000	3.000
Rmerge	0.148	1.056
Number of reflections	16932	1670
<I/σ(I)>	11.25
Completeness [%]	99.8	99.7
Redundancy	6
CC(1/2)	0.994	0.765

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291.15	500mM sodium chloride, 10% (w/v) PEG 4000, 50mM Tris-HCl