6Q0N
Structure of the Erbin PDB domain in complex with a high-affinity peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.060, 35.359, 38.676 |
| Unit cell angles | 83.88, 75.48, 68.99 |
Refinement procedure
| Resolution | 30.040 - 1.180 |
| R-factor | 0.158 |
| Rwork | 0.155 |
| R-free | 0.18300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SWISS-MODELLER model of the E-14 variant of the Erbin PDZ |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.040 | 1.180 |
| High resolution limit [Å] | 1.160 | 1.160 |
| Rmerge | 0.051 | 0.517 |
| Number of reflections | 47892 | 1996 |
| <I/σ(I)> | 12.4 | 1.8 |
| Completeness [%] | 86.7 | 71.5 |
| Redundancy | 3.5 | 3.3 |
| CC(1/2) | 0.996 | 0.856 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 6%PEG3350, 100 mM MgCl2, 100 mM Sodium Acetate pH 4.5. Cryoprotection was achieved with the same buffer plus 25% ethylene glycol |
