6Q0L

Inferred intermediate I-7 (I-7-1) of the human antibody lineage 652 in complex with influenza hemagglutinin head domain of A/Beijing/262/95(H1N1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	80
Detector technology	CCD
Collection date	2018-06-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	57.590, 70.280, 191.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.340 - 2.500
R-factor	0.2191
Rwork	0.217
R-free	0.25970
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.340	2.589
High resolution limit [Å]	2.500	2.500
Rmerge	0.075	1.002
Number of reflections	27683	2708
<I/σ(I)>	18.34
Completeness [%]	99.9
Redundancy	7.1
CC(1/2)	0.999	0.814

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	100 mM ammonium sulfate, 20% (w/v) PEG 4000, 100 mM CHES