6PYL
Crystal Structure of HLA-B*2703 in complex with KK10, an HIV peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.847, 82.649, 104.755 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.400 - 1.520 |
| R-factor | 0.203 |
| Rwork | 0.201 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g9d |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.240 | 1.550 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rpim | 0.040 | 0.423 |
| Number of reflections | 68648 | 24151 |
| <I/σ(I)> | 14.5 | 2.1 |
| Completeness [%] | 100.0 | |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 277 | 20-30% PEG 4K, 0.2M Na Acetate and 0.1M Na Citrate pH 5.6 |
