6PY2
HLA-TCR complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.953700 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 75.280, 275.140, 141.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.857 - 2.825 |
| R-factor | 0.2173823465 |
| Rwork | 0.216 |
| R-free | 0.24416 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ozh |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.746 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.860 | 2.930 |
| High resolution limit [Å] | 2.820 | 2.820 |
| Number of reflections | 35664 | 462 |
| <I/σ(I)> | 10.95 | |
| Completeness [%] | 85.0 | |
| Redundancy | 2 | |
| CC(1/2) | 0.998 | 0.854 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M MES, pH 6.5, 5% v/v MPD, 15% w/v PEG6000 |
