6PXO

Human Casein Kinase 1 delta (anion-free crystallization conditions)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-18
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.11583
Spacegroup name	P 1 21 1
Unit cell lengths	49.910, 130.490, 51.160
Unit cell angles	90.00, 112.75, 90.00

Refinement procedure

Resolution	65.240 - 2.000
R-factor	0.1819
Rwork	0.179
R-free	0.23750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5x17
RMSD bond length	0.008
RMSD bond angle	0.965
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.240	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.100
Rpim	0.045	0.468
Number of reflections	40494	5884
<I/σ(I)>	11.2	1.7
Completeness [%]	99.6	99.7
Redundancy	6.2	5.8
CC(1/2)	0.990	0.610

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	295	0.15 M Succinic Acid 17% PEG 3350