6PX6
HLA-TCR complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-05-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.953728973866 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.534, 99.416, 72.565 |
| Unit cell angles | 90.00, 118.26, 90.00 |
Refinement procedure
| Resolution | 39.238 - 3.000 |
| R-factor | 0.207648302688 |
| Rwork | 0.205 |
| R-free | 0.25702 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ozh |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.595 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.240 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 18263 | 18263 |
| <I/σ(I)> | 4.21 | |
| Completeness [%] | 99.9 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 0.1 M MOPS/HEPES, pH 7.5, 12.5% v/v PEG1000, 12.5% v/v MPD, 12.5% w/v PEG3350, 0.04 M NPS mixture |
