6PWT

Crystal structure of the cow C-type carbohydrate-recognition domain of CD23 in the presence of GlcNAc2Man3 oligosaccharide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 31 2 1
Unit cell lengths	33.167, 33.167, 207.814
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.636 - 2.701
R-factor	0.201
Rwork	0.199
R-free	0.25090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pws
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.640	2.876
High resolution limit [Å]	2.700	2.700
Rmerge	0.105	0.344
Rmeas	0.108	0.359
Rpim	0.027	0.100
Total number of observations	70226	6699
Number of reflections	4156	518
<I/σ(I)>	13.7	3.1
Completeness [%]	99.5	96.9
Redundancy	16.9	12.9
CC(1/2)	0.996	0.985

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	290	protein solution: 5 mg/ml protein, 5 mM CaCl2, 10 mM Tris-Cl, pH 8.0, 25 mM NaCl, and 10 mM GlcNAc2Man3. reservoir solution: 22% polyethylene glycol 8K, 0.1 M HEPES pH 7.0