6PWD
Ewingella americana HopBF1 kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-16 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0070 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 134.953, 134.953, 124.489 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.600 - 2.470 |
| R-factor | 0.2035 |
| Rwork | 0.202 |
| R-free | 0.21750 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.556 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.600 | 2.510 |
| High resolution limit [Å] | 2.470 | 2.470 |
| Rmerge | 0.054 | 1.208 |
| Rpim | 0.013 | 0.304 |
| Number of reflections | 15724 | 770 |
| <I/σ(I)> | 55.8 | 2.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 18.1 | 16.4 |
| CC(1/2) | 0.800 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 1.4 M ammonium sulfate, 0.1 M sodium citrate, 0.2 M sodium potassium tartrate, 0.05 M sodium chloride, 25% ethylene glycol |
