6PVX
RNase A in complex with p5U
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER IMUS MICROFOCUS |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-29 |
| Detector | Bruker PHOTON III |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.540, 32.640, 72.170 |
| Unit cell angles | 90.00, 90.44, 90.00 |
Refinement procedure
| Resolution | 29.420 - 1.550 |
| R-factor | 0.2008 |
| Rwork | 0.199 |
| R-free | 0.22810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1afu |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.420 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.060 | 0.060 |
| Rmeas | 0.062 | 0.017 |
| Rpim | 0.017 | 0.062 |
| Number of reflections | 34001 | 2948 |
| <I/σ(I)> | 25.4 | 3 |
| Completeness [%] | 98.7 | 87.1 |
| Redundancy | 12.1 | 3 |
| CC(1/2) | 1.000 | 0.823 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 20 mM sodium citrate, 20% (v/v) PEG 4000 |
