6PVG

Crystal structure of ligand free PhqK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.033
Spacegroup name	P 21 21 21
Unit cell lengths	47.724, 84.210, 116.389
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.875 - 1.709
R-factor	0.1635
Rwork	0.162
R-free	0.19200
Structure solution method	MOLECULAR REPLACEMENT
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.880	1.770
High resolution limit [Å]	1.700	1.710
Number of reflections	630819	4603
<I/σ(I)>	1.34
Completeness [%]	99.0
Redundancy	12.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	25% PEG 3350, 200 mM ammonium acetate, 100 mM Bis-Tris pH 5.5, 2% 2,2,2-trifluoroethanol