6PUK
Structure of human MAIT A-F7 TCR in complex with human MR1-JYM72
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 217.055, 69.688, 143.319 |
| Unit cell angles | 90.00, 104.35, 90.00 |
Refinement procedure
| Resolution | 52.570 - 2.080 |
| R-factor | 0.1769 |
| Rwork | 0.175 |
| R-free | 0.20170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4l4t |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.523 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.150 | 2.120 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.056 | 0.857 |
| Number of reflections | 124837 | 6219 |
| <I/σ(I)> | 13.57 | |
| Completeness [%] | 99.9 | |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM BTP (pH 6.0 - 6.5), 8-20% PEG3350 and 200 mM sodium acetate |
