6PU4

MicroED structure of proteinase K recorded on Falcon III

Experimental procedure

Spacegroup name	P 43 21 2
Unit cell lengths	67.480, 67.480, 101.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.660 - 2.100
Rwork	0.221
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5k7s
RMSD bond length	0.008
RMSD bond angle	1.472
Data reduction software	MOSFLM (7.2.2)
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP (11.7.01)
Refinement software	REFMAC (5.8.0238 2018/15/10)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.830
High resolution limit [Å]	2.700	2.700
Rmerge		1.972
Number of reflections		825
<I/σ(I)>		0.9
Completeness [%]		97.2
Redundancy		3.8
CC(1/2)		0.169