6PU2

Dark, Mutant H275T , 100K, PCM Myxobacterial Phytochrome, P2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2018-07-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.03
Spacegroup name	P 1 21 1
Unit cell lengths	82.405, 81.790, 83.493
Unit cell angles	90.00, 107.00, 90.00

Refinement procedure

Resolution	42.240 - 2.200
Rwork	0.219
R-free	0.28960
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-3000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Number of reflections	45897	2049
<I/σ(I)>	184.5
Completeness [%]	100.0
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	Ammonium acetate, Sodium citrate tribasic dihydrate, PEG 4000, Benzamidine Hydrochloride