6PTX
Dark, 100K, PCM Myxobacterial Phytochrome, P2, Wild Type,
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-06-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.03 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.048, 81.328, 83.107 |
| Unit cell angles | 90.00, 107.13, 90.00 |
Refinement procedure
| Resolution | 41.990 - 1.650 |
| Rwork | 0.198 |
| R-free | 0.22840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.690 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 121358 | 7971 |
| <I/σ(I)> | 162.2 | |
| Completeness [%] | 92.0 | 84.1 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | Ammonium acetate, Sodium citrate tribasic dihydrate, PEG 4000, Benzamidine Hydrochloride |
