6PTM
Crystal structure of apo exo-carrageenase GH42 from Bacteroides ovatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-05 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1.1271 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.240, 109.690, 126.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.340 - 2.000 |
| R-factor | 0.1672 |
| Rwork | 0.165 |
| R-free | 0.20090 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.631 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.660 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.128 | 0.682 |
| Number of reflections | 57229 | 4129 |
| <I/σ(I)> | 11.2 | 2.4 |
| Completeness [%] | 99.9 | |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | PEG 400, Tris, MgCl2 |
