6PSD
Complex of CRACR2a with a Dynein Light Intermediate Chain Peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.290, 161.990, 56.900 |
| Unit cell angles | 90.00, 104.01, 90.00 |
Refinement procedure
| Resolution | 43.350 - 2.660 |
| R-factor | 0.2008 |
| Rwork | 0.197 |
| R-free | 0.23240 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.505 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.352 | 2.780 |
| High resolution limit [Å] | 2.660 | 2.660 |
| Rmerge | 0.359 | 0.953 |
| Rpim | 0.114 | 0.330 |
| Number of reflections | 28181 | 2798 |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 99.9 | |
| Redundancy | 10.8 | |
| CC(1/2) | 0.960 | 0.440 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 16% w/v polyethylene glycol 10,000, 0.5 M Bis-Tris, pH 6.5, and 10 mM CoCl2 |
