6PRV

58nt RNA L11-binding domain from E. coli 23S rRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-01-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97625
Spacegroup name	C 1 2 1
Unit cell lengths	89.610, 73.610, 130.280
Unit cell angles	90.00, 100.96, 90.00

Refinement procedure

Resolution	39.360 - 2.710
R-factor	0.2067
Rwork	0.204
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hc8
RMSD bond length	0.005
RMSD bond angle	1.141
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.870
High resolution limit [Å]	2.710	2.710
Rmeas	0.086	2.360
Number of reflections	22407	3442
<I/σ(I)>	12.92	4.9
Completeness [%]	97.9
Redundancy	5.1
CC(1/2)	0.990	0.490

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	5-20 % PEG 4000, 0.6-1.2M NaCl and Sodium cacodylate (PH6.5)