6PRE
SBP RafE in complex with verbascose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-21 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54187 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.918, 145.877, 118.371 |
| Unit cell angles | 90.00, 93.64, 90.00 |
Refinement procedure
| Resolution | 29.550 - 2.400 |
| R-factor | 0.2332 |
| Rwork | 0.231 |
| R-free | 0.27130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6prg |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.645 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.152 | 0.530 |
| Rpim | 0.076 | 0.344 |
| Number of reflections | 64353 | 4289 |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 99.6 | |
| Redundancy | 4 | |
| CC(1/2) | 0.984 | 0.706 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 0.1M MnCl2, 0.2M CsCl, 0.1M Tris, 20% w/v PEG 3350 |
