6PRC

PHOTOSYNTHETIC REACTION CENTER FROM RHODOPSEUDOMONAS VIRIDIS (DG-420314 (TRIAZINE) COMPLEX)

Experimental procedure

Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	263
Detector technology	IMAGE PLATE
Collection date	1993-08
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	223.500, 223.500, 113.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.184
Rwork	0.184
R-free	0.22100 *
Structure solution method	DIFFERENCE FOURIER / SA OMIT MAPS
Starting model (for MR)	2prc
RMSD bond length	0.011
RMSD bond angle	23.700 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((ROTAVATA)
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000 *	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.073	0.247
Total number of observations	256827 *
Number of reflections	102117 *
<I/σ(I)>	9.1	2.7
Completeness [%]	81.4 *	56.6 *
Redundancy	2.5	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6		pH 6.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ammonium sulfate	1.5 (M)
2	1	reservoir	ammonium sulfate	3 (M)	or 2.6M or 2.4M