6PQN

Crystal structure of HzTransib transposase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792, 0.9197, 1.4586, 1.1398, 1.0718, 1.0087
Spacegroup name	P 61 2 2
Unit cell lengths	160.292, 160.292, 235.858
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	80.150 - 3.010
R-factor	0.2222
Rwork	0.219
R-free	0.27700
Structure solution method	MIRAS
RMSD bond length	0.004
RMSD bond angle	0.750
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	200.000	3.117
High resolution limit [Å]	3.010	3.010
Rmerge	0.086	2.012
Rmeas	0.093	2.160
Rpim	0.033	0.772
Number of reflections	35894	3455
<I/σ(I)>	14.47	0.66
Completeness [%]	99.2	98.2
Redundancy	7.7	7.6
CC(1/2)	0.999	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	100 mM HEPES, pH 7.0, 0.7-0.8 M NaH2PO4, 0.75 M KH2PO4