6PQ7
Structure of the iMango-III fluorescent aptamer at room temperature.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SLAC LCLS BEAMLINE MFX |
| Synchrotron site | SLAC LCLS |
| Beamline | MFX |
| Temperature [K] | 296 |
| Detector technology | PIXEL |
| Collection date | 2018-04-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.303 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 53.560, 53.560, 194.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.640 - 3.000 |
| R-factor | 0.2726 |
| Rwork | 0.269 |
| R-free | 0.30440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6e8u |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.141 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.500 | 3.300 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 5419 | 368 |
| <I/σ(I)> | 2.3 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3 | 3 |
| CC(1/2) | 0.700 | 0.245 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 294 | 0.1 M Na cacodylate pH 7.1, 17.5% (w/v) polyethylene glycol (PEG) 3350, and 0.25 M Magnesium acetate |
