6PPM
Ancestral Caspase 6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-06-19 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.314, 88.542, 141.026 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.240 - 2.610 |
| R-factor | 0.182 |
| Rwork | 0.177 |
| R-free | 0.25090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j30 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.911 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.240 | 2.700 |
| High resolution limit [Å] | 2.610 | 2.610 |
| Number of reflections | 32724 | 3134 |
| <I/σ(I)> | 5.1 | |
| Completeness [%] | 99.7 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.951 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.2 M ammonium fluoride, pH 6.2, 20% PEG 3350 |
