6POK

Crystal structure of the Robo3 FN2-3 domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	120
Detector technology	PIXEL
Collection date	2017-06-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 1 21 1
Unit cell lengths	54.986, 36.607, 64.896
Unit cell angles	90.00, 114.44, 90.00

Refinement procedure

Resolution	50.060 - 1.796
R-factor	0.2185
Rwork	0.217
R-free	0.24310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hlj
RMSD bond length	0.002
RMSD bond angle	0.531
Data reduction software	XDS (20170601)
Data scaling software	XDS (20170601)
Phasing software	MoRDa (1.3.02, database v. 20)
Refinement software	PHENIX ((1.14rc1_3177: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.000	1.910
High resolution limit [Å]	1.796	1.796
Rmerge	0.066	0.860
Rmeas	0.092	1.207
Number of reflections	21801	5642
<I/σ(I)>	6.59
Completeness [%]	91.2	80.8
Redundancy	1.8	1.6
CC(1/2)	0.996	0.586

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.1 M HEPES, pH 7.5, 12% PEG 6,000