6PNU
Crystal structure of native DauA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-14 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.880, 137.800, 108.870 |
| Unit cell angles | 90.00, 109.94, 90.00 |
Refinement procedure
| Resolution | 75.664 - 2.000 |
| R-factor | 0.2234 |
| Rwork | 0.222 |
| R-free | 0.25890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3jz4 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.664 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.146 | 0.656 |
| Rpim | 0.075 | 0.346 |
| Number of reflections | 153728 | 7558 |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 99.4 | 98.9 |
| Redundancy | 5.3 | 5.3 |
| CC(1/2) | 0.984 | 0.818 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.3 M ammonium fluoride, 25 % PEG 3350 |
