6PNQ

Crystal structure of the SS2A splice insert-containing neurexin-1 LNS2 domain in complex with neurexophilin-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	C 1 2 1
Unit cell lengths	71.962, 61.443, 79.392
Unit cell angles	90.00, 106.22, 90.00

Refinement procedure

Resolution	45.920 - 1.947
R-factor	0.2007
Rwork	0.196
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pnp
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.920	2.020
High resolution limit [Å]	1.947	1.950
Rmerge	0.108	1.421
Rpim	0.031	0.529
Number of reflections	23404	583
<I/σ(I)>	11.4	0.78
Completeness [%]	83.2	24.18
Redundancy	12	6.3
CC(1/2)	0.998	0.559

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	16% PEG 3350, 2% Tacsimate, and 0.1 M sodium citrate tribasic