6PMT

Structure of Mortalin-NBD with adenosine-5'-monophosphate and thiodiphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000074
Spacegroup name	P 21 21 21
Unit cell lengths	52.829, 56.548, 141.363
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.486 - 2.300
R-factor	0.1924
Rwork	0.191
R-free	0.20540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6nhk
RMSD bond length	0.008
RMSD bond angle	1.209
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.490	2.382
High resolution limit [Å]	2.300	2.300
Rmerge	0.066	0.166
Rmeas	0.072
Number of reflections	19382	1811
<I/σ(I)>	21.78
Completeness [%]	98.1
Redundancy	6.6
CC(1/2)	0.998	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	298	100 mM sodium acetate, 20% (w/v) PEG 3000