6PL8

Structure of human DNA polymerase eta complexed with 8OA in the template base paired with incoming non-hydrolyzable TTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9765
Spacegroup name	P 61
Unit cell lengths	98.705, 98.705, 81.719
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.870 - 2.170
R-factor	0.1848
Rwork	0.182
R-free	0.24380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4o3n
RMSD bond length	0.009
RMSD bond angle	1.295
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.210
High resolution limit [Å]	2.170	2.170
Number of reflections	24030	1190
<I/σ(I)>	18.4
Completeness [%]	100.0
Redundancy	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1M MES (pH 5.5), 5 mM MgCl2, and 15 to 20 % PEG 2K-MME