6PL7
Structure of human DNA polymerase eta complexed with A in the template base paired with incoming non-hydrolyzable TTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9765 |
| Spacegroup name | P 61 |
| Unit cell lengths | 98.700, 98.700, 82.447 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.700 - 2.500 |
| R-factor | 0.2136 |
| Rwork | 0.210 |
| R-free | 0.28080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o3n |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.280 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 15946 | 790 |
| <I/σ(I)> | 15.3 | |
| Completeness [%] | 99.8 | |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1M MES (pH 5.5), 5 mM MgCl2, and 15 to 20 % PEG 2K-MME |
