6PL6
Structural coordination of polymerization and crosslinking by a peptidoglycan synthase complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 118.440, 118.440, 265.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.235 - 3.300 |
| R-factor | 0.2769 |
| Rwork | 0.275 |
| R-free | 0.30760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6pl5 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.235 | 3.390 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.243 | |
| Number of reflections | 59327 | 2411 |
| <I/σ(I)> | 5.86 | 0.58 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 6.77 | 6.82 |
| CC(1/2) | 0.999 | 0.330 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 6.6 | 293 | Protein complex was reconstituted with monoolein. Crystals were grown in 100 mM MES pH 5.8-6.8, 100 mM Na2SO4, 10 mM SrCl2, 35-45% PEG 300 |
