6PL5

Structural coordination of polymerization and crosslinking by a peptidoglycan synthase complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033
Spacegroup name	P 32 2 1
Unit cell lengths	119.570, 119.570, 267.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.766 - 3.500
R-factor	0.2869
Rwork	0.285
R-free	0.30530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bar 3lo7
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.766	3.590
High resolution limit [Å]	3.500	3.500
Rmerge	0.159
Number of reflections	50777	2071
<I/σ(I)>	8.15	0.62
Completeness [%]	99.3	99.8
Redundancy	6.11	6.11
CC(1/2)	0.999	0.334

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6.4	293	Protein complex was reconstituted with monoolein. Crystals were grown in 100 mM MES pH 5.8-6.5, 100 mM Li2SO4, 40-50% PEG 300