6PJP
Time-resolved structural snapshot of proteolysis by GlpG inside the membrane
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.977 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 73.029, 98.560, 62.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.720 - 2.450 |
| R-factor | 0.25163 |
| Rwork | 0.242 |
| R-free | 0.27130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5f5d |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.497 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.750 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.275 | |
| Number of reflections | 8800 | 948 |
| <I/σ(I)> | 6.4 | |
| Completeness [%] | 95.3 | |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1 M Na-acetate pH 5.5, 3 M NaCl, and 5 % ethylene glycol |
