6PIR

Crystal structure of the Legionella effector protein MavE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	293.15
Detector technology	PIXEL
Collection date	2016-11-26
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	199.550, 42.050, 46.270
Unit cell angles	90.00, 90.66, 90.00

Refinement procedure

Resolution	46.267 - 1.650
R-factor	0.1968
Rwork	0.195
R-free	0.23740
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.151
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AutoSol
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.770	1.690
High resolution limit [Å]	1.650	1.650
Number of reflections	82310	13055
<I/σ(I)>	15.19
Completeness [%]	99.3
Redundancy	6.96
CC(1/2)	0.999	0.593

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1M Citrate, pH 6.0, 18% PEG20K