6PIM

Crystal Structure of Human Protocadherin-1 EC3-4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-30
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97921
Spacegroup name	P 42 21 2
Unit cell lengths	125.569, 125.569, 45.294
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.430 - 3.050
R-factor	0.22201
Rwork	0.220
R-free	0.26614
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dzv
RMSD bond length	0.009
RMSD bond angle	1.536
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.100
High resolution limit [Å]	3.050	3.050
Rmerge	0.166	1.027
Rmeas	0.175	1.110
Rpim	0.054	0.401
Number of reflections	7363	319
<I/σ(I)>	12	1.67
Completeness [%]	99.3	90.6
Redundancy	9.9	6.5
CC(1/2)	0.938	0.551

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	0.1 M MES pH 6.5, 20% PEG 4000, 0.6 M Sodium Chloride